The relationship of mRNA with protein expression in CD8+ T cells associates with gene class and gene characteristics
Fig 5
ARE clusters differentially modulate mRNA and protein levels in resting and activated CD8+ T cells.
(A-B) mRNA (TPM) and protein (CN) abundance in (A) CD45RO+ CD8+ memory T cell subsets (TMEM; n = 16; from [22]) and (B) TActivated CD8+ T cells of all genes, grouped according to the number of AU-rich element in cluster within the transcript’s 3’UTR clusters according to ARED-plus annotation (A; see methods). Differences between groups were assessed with a two-tailed t-test followed by adjustment of p-value using the Benjamini-Hochberg procedure. Adjusted p-value are indicated. (C) and integrated mRNA and protein abundance. The slope of the regression line of each group is color-coded in (A, B), and only gene products that are detected both at mRNA and protein levels are shown. TPM: Transcripts per kilo-base per million; CN: Protein copy number. n.s.: not significant. “*” indicates correlation and regression with p-value <0.05.
