A Modular Cloning System for Standardized Assembly of Multigene Constructs
Figure 7
Structure of the eleven level 1 modules (A) and the final level 2 construct cL2-13 (B).
All transcription units were assembled from 5 plasmids: 4 level 0 modules (promoter, 5′ untranslated region, CDS, and terminator) and a destination vector. All proteins are cytosolic except the two from constructs cL1-8 and 9 which are secreted. For both of these, the coding sequence module already contained the signal peptide. LB, T-DNA left border; RB, T-DNA right border; Ω, tobacco mosaic virus 5′ untranslated region; genetic elements used are listed in Table S1.
