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Evaluation tools that measure possible damage to the environment and human health are needed to develop greener products, technologies and services. Now, a life cycle assessment protocol introduces a stepwise process for assessing the environmental impact of a variety of products such as automotive tires as well as of infrastructure, healthcare and more.
Life cycle assessment quantifies environmental impacts during the full life cycle of a product or process. This tutorial uses ReCiPe to perform the impact assessment phase, where the magnitude and significance of environmental impacts are evaluated.
We present best practice guidelines for the use of concurrent TMS-fMRI, experimental design, technical requirements and data interpretation, illustrated via 12 use cases.
Nanoparticle pharmacokinetics is affected by the formation of a protein corona in vivo. Mass spectrometry-based proteomics is essential for understanding the corona composition. This Perspective discusses how method reproducibility can be improved.
In this Tutorial, we provide guidelines for the use of ML models to mine genomes and proteomes for the discovery of antimicrobial peptides, discussing the various stages of the ML workflow, recent advances in ML models and how to experimentally validate the computational predictions.
In this Tutorial, the authors provide guidelines for quality control of whole-exome and whole-genome sequencing data for population-scale association analyses. They explain how to filter samples and variants, and compare commonly used software programs for quality control of sequencing data.
CellPhoneDB infers cell–cell communication by integrating single-cell genomics data with a curated database of ligand–receptor interactions. Now, a Protocol Update describes the latest version of the tool including many new features.
Now a protocol enables simultaneous measurement of gene expression and chromatin state in intact tissues, unlocking the relationship between tissue architecture and gene regulation.
Single-cell CRISPR screening is a powerful method for biological discovery. Such screens genetically perturb many genes in parallel and use single-cell RNA sequencing to assess the transcriptomic effects. Now, an in vivo protocol facilitates screens in mouse brain, using adeno-associated virus with transposon-based delivery of the CRISPR guide RNAs.
A protocol for the preparation of crystals for microcrystal electron diffraction provides X-ray crystallographers and cryo-electron microscopists access to a method that facilitates the determination of molecular structures from submicrometer crystals at atomic resolution.
Poorly performing antibodies contribute to the reproducibility crisis in biomedical research. The market offers millions of antibodies and validating them is a cumbersome process. Now, the non-profit Antibody Characterization through Open Science (YCharOS) organization shares a step-by-step protocol for rigorous antibody validation using genetic (knockout) cell line controls.
A recent protocol for PASTE (programmable addition via site-specific targeting elements) combines prime editing and site-specific integrases to integrate large genetic payloads.
Standardized neuroimaging protocols are essential to advancing comparability and replicability. Now, a comprehensive magnetic resonance imaging acquisition and analysis protocol from the Rhineland Study provides a valuable framework for image acquisition and analysis that is suitable for both large- and small-scale neuroimaging studies.
The members of Independent SAGE reflect on their experience in setting up, developing and running a science communication platform and service to the public during the COVID-19 pandemic
This tutorial describes a complete pipeline for structural connectomics, including fundamental aspects of diffusion MRI and its applications for exploring structural connectomics, including fiber tracking and constructing a structural network.
A Tutorial review on the measurement of membrane mechanical forces reported via fluorescence lifetime variations induced by conformational changes of the Flipper probes.
This Consensus Statement, which presents the best practices for implantation, monitoring and assessment of tumors in rodents, aims to improve the refinement of cancer models in vivo.
A consensus statement outlines the best practices to refine animal welfare in rodent models of cancer. Their adoption will allow the standardization of experimental interventions, considering the characteristics of each cancer model, thereby improving the reproducibility and translational value of data obtained in rodents.
This tutorial provides guidelines on oncolytic virus design, production and testing in cancer immunotherapy. Best practice recommendations for preclinical and clinical use of oncolytic viruses as an immunotherapy tool and related future challenges are also considered.
