Extended Data Fig. 10: Single cell whole genome sequencing of second time points.
From: Tracking clonal evolution during treatment in ovarian cancer using cell-free DNA
a) Clonal tracking in patient 046 b) patient 026 and c) patient 139. For each patient we show the anatomical sites sequenced with DLP, a phylogenetic tree of the clones, clonal fractions, mean truncal SV VAF and TP53 VAF from cfDNA, CA-125 and treatment history over time. For these patients, scWGS was generated from a second timepoint, anatomical site and time of second time point are annotated on the anotamical diagram and at the top of the clone fraction plot (S1 vs S2). Comparison of pre-and post treatment scWGS data for these 3 patients: d) 026 f) 139 and g) 046. For each panel we show the phylogenetic tree of all cells annotated by clone, timepoint (pre or post treatment) and whole genome doubled (WGD) state. Then the consensus total copy number heatmap for each clone based on pre-treatment cells and the total copy number heatmap for all post-treatment cells. e) Shows a pre-treatment WGD cell from clone C in 026 and the consensus copy number profile of all post-treatment cells. Illustrations in a-c were created using BioRender (https://biorender.com).