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Isolation of Cytoskeleton and Cytoskeleton-Bound Polysome, and Identification of Cytoskeletal Proteins from a Grain Legume
The plant cytoskeleton is an essential component of cellular architecture, enabling various critical metabolic processes, including cell division,...
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CRISPR/Cas9-Based Gene Editing in Soybean
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated Cas9)-based gene editing is a robust tool for functional...
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Immunohistochemical Localization and Anatomical Distribution of Enteric Glia Cells in Enteric Nervous System
The enteric nervous system (ENS) comprises highly heterogeneous glia cell populations, which play critical roles in the regulation of intestinal...
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Local Haplotyping Analysis for Flowering Time in Soybean Using Crosshap
The vast amount of genomic data available for various plant species has enabled a greater understanding of genomic regions associated with phenotypic...
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Identification of tRNA-Derived Fragments in Legumes
The tRNA-derived noncoding RNAs (tncRNAs) belong to the novel class of noncoding RNAs, acting as important components of genome regulatory circuits....
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A Computational Pipeline for the Identification of RNA Virome in Legumes
Viruses are the most diverse biological entities ranging from simple forms with just a few genes to complex forms with hundreds of proteins. Among...
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A Multi-omics Approach for Microbiome Data Analysis in Legumes
Microbiome plays a crucial role in influencing the health and function of living beings as well as in regulating the biogeochemical cycles. The plant...
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Isolation of Distinct Populations of Enteric Glia from the Muscularis Externa and Mucosa of the Mouse Intestine
Enteric glia, integral to the enteric nervous system (ENS), are crucial for intestinal motility, secretomotor function, and immunity. These glia...
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Comprehensive Method for Soybean Cultivar Identification Using MGIseq, VCF2GENOME, and REST API
This protocol provides a comprehensiveREST APIMGIseqCultivar identificationSoybean method for identifying soybeanSoybeans (Glycine max) cultivars...
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Large-Scale Identification of Transposon Insertion Polymorphisms in Leguminous Plants from Paired-End WGS Data
Transposable elements (TEs) are dynamic genomic sequences that significantly influence genome architecture and function. Their ability to move and...
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Legume Pangenome Construction Using an Iterative Mapping and Assembly Approach
A pangenomePangenomes is a collectionPangenomes of genomic sequences found in the entire species rather than a single individual. It allows...
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Rodent Enteric Glia Culture
Enteric glia play fundamental roles in the plasticity and homeostasis of the enteric nervous system. Disruptions in their function contribute to...
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Immunohistochemical Staining Protocol of Enteric Glial Cells in Myenteric Plexi
Despite the development of several genetic approaches to visualize enteric glia within the enteric nervous system (ENS), multiple fluorescence...
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Pichia pastoris Continuous Cultivations for Strain Characterization and Continuous Biomanufacturing
Continuous cultivation provides physiological and product-related data of the recombinant-producing strain, which is crucial for bioprocess design...
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Oligo-dT Anchored cDNA SCoT: An Innovative Approach for Deciphering Fusarium Wilt Resistance-Related Differential Gene Expression in Chickpea
The cDNA start codon targeted marker (cDNA-SCoT) methodology is a unique, simple, quick, and efficient method for investigating differential gene...
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A Blotting Paper Technique for the Screening of Chickpea Genotypes Against Dry Root Rot Disease
Dry root rot (DRR) disease is a major threat to chickpea production across the world. This disease is caused by a soil-borne necrotrophic fungal...
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A Guide to Culturing, Maintenance, and Leaf Inoculation Methods for Rapid Screening and Quantification of Sclerotinia sclerotiorum Infection in Mustard
Sclerotinia rot poses a significant challenge to the cultivation of oilseed Brassica crops, causing the reduction of seed number, weight, and quality...
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Autonomously Replicating Sequence-Bearing Plasmids Utilized in Pichia pastoris
Plasmids are a common tool in biotechnology to deliver recombinant DNA into microbial cells for the production of enzymes, pharmaceutical proteins,...
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In Vivo Plasmid Assembly in NHEJ-Deficient Pichia pastoris Strains
The integration of linear plasmids into the genome is the most common method for genetic engineering of Komagataella phaffii (reclassified from...
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Overcoming the Challenges of Producing Plant Phytochromes in Escherichia coli
Biochemical and structural studies of plant phytochromes have been limited until recently by the difficulty of producing them in large amounts and as...
