Extended Data Fig. 2: RNA-Seq data of MitoTracker-high and -low HPFs in co-cultures.
a. FACS gating strategy for isolation of HPFs from A431-HPF co-cultures, demonstrating the arbitrary division into two HPF populations. FSC: Forward scatter; SSC; side scatter. b. Principal Component Analysis (PCA) of RNA-seq data from MitoTracker-high and -low HPFs and control HPFs that underwent the sorting procedure but were not maintained in co-cultures. n = 3 co-cultures per group. c, d. Volcano plots showing differentially expressed genes in HPFs in co-culture (MitoTracker-high (c) or -low (d)) vs. control HPFs in monoculture. Selected CAF marker genes are in red. n = 3 co-cultures and sorting experiments. e. RNA-seq data showing expression of ISGs in MitoTracker -high and -low HPFs after co-culture with A431 cells vs. control HPFs. n = 3 co-cultures per group. f. qRT-PCR for INHBA and IL6 relative to RPL27 using RNA from MitoTracker-high and -low HPFs after co-culture with A431 cells vs. control HPFs (repetition experiment). n = 3 co-cultures per group. Graphs show mean ± SEM. Unpaired two-sided Student’s t-test (f) or two-sided one-way ANOVA with Bonferroni post-host multiple comparison test (e) were used to determine statistical significance.