Extended Data Fig. 4: Metabolite profiles in nATF6IEC mice.
From: ATF6 activation alters colonic lipid metabolism causing tumour-associated microbial adaptation
a, PCA of metabolite profiles comparing fl/fl (circles), tg/wt (squares) and tg/tg (triangles) mice at 5wk, 12wk and 20wk. Grey indicates non-tumour (NT) and red tumour (T) (5wk n = 5 fl/fl mice, n = 6 tg/wt mice, n = 7 tg/tg mice; 12wk n = 6 fl/fl mice, n = 6 tg/wt mice, n = 6 tg/tg mice, 20wk n = 6 fl/fl mice, n = 6 tg/wt mice, n = 5 tg/tg mice). b, Volcano plot of differentially enriched metabolites between fl/fl and tg/tg mice across all time points (Log2 fold change threshold = 1.0, FDR threshold = 0.1). Significance was calculated using paired T-tests on log-transformed data (5wk n = 5 fl/fl mice, n = 6 tg/wt mice, n = 7 tg/tg mice; 12wk n = 6 fl/fl mice, n = 6 tg/wt mice, n = 6 tg/tg mice, 20wk n = 6 fl/fl mice, n = 6 tg/wt mice, n = 5 tg/tg mice). c, PCA of luminal lipid profiles between tumor-susceptible/tumor (T) and non-tumor (NT) mice at 5wk, 12wk and 20wk (5wk n = 5 fl/fl mice, n = 6 tg/wt mice, n = 7 tg/tg mice; 12wk n = 6 fl/fl mice, n = 6 tg/wt mice, n = 6 tg/tg mice, 20wk n = 6 fl/fl mice, n = 6 tg/wt mice, n = 5 tg/tg mice). d, Heatmap of scaled (z-score) differentially enriched metabolites, sorted by genotype and age. Rows are clustered using Ward’s method (5wk n = 5 fl/fl mice, n = 6 tg/wt mice, n = 7 tg/tg mice; 12wk n = 6 fl/fl mice, n = 6 tg/wt mice, n = 6 tg/tg mice, 20wk n = 6 fl/fl mice, n = 6 tg/wt mice, n = 5 tg/tg mice).