Extended Data Fig. 7: Stress granules, proteasomal and lysosomal activities are increased in Tex cells.
From: Proteotoxic stress response drives T cell exhaustion and immune evasion
All experiments were performed using mouse Teff and Tex cells generated in vitro 8 days post initial activation. a, ImageStream analysis of G3BP1 stress granules (SGs) in Teff and Tex cells. b, Quantification of the frequency of G3BP1 SGs in Teff and Tex cells determined with Bright Detail Intensity by ImageStream analysis. At least 2,000 live cells were examined for each replicate (n = 4, two-tailed t test). c, Flow cytometry quantification of G3BP1 expression in Teff and Tex cells (n = 4, two-tailed t test). d, Representative flow cytometry plots and statistics of G3BP1 expression in G3bp1 knockout and control wild-type Tex cells (n = 4, two-tailed t test). e, Representative flow cytometry plots and statistics of the percentage of IFNγ+TNFα+ cells out of the total CD8+ T cells in G3bp1 knockout and wild-type Tex cells (n = 4, two-tailed t test). f, Viability of G3bp1 knockout and wild-type Tex cells (n = 4, two-tailed t test). g, Proteasomal activity in Teff and Tex cells (n = 4, two-tailed t test). h, Flow cytometry quantification of lysosome mass in Teff and Tex cells (n = 6, two-tailed t test). i, Degradation kinetics of newly synthesized proteins in Teff and Tex cells (n = 3). j, Fold changes of HPG MFI 8 h post “pulsing” with MG132, Bafilomycin A1(BafA1) or DMSO in Tex cells (n = 5, one-way ANOVA). Data in b-h and j are presented as mean ± SD. Data in i are presented as mean ± SEM.